Teneurin-2 Antibody [Unconjugated]

Western blot shows lysates of mouse brain tissue and rat embryonic cortical neuron cells. PVDF Membrane was probed with 1 µg/mL of Human Teneurin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4578) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin-2 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Teneurin-2 was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Human/Mouse/Rat Teneurin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4578) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in dorsal root ganglia and cells in cartilage primordium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of U251-MG human malignant glioblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Teneurin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4578) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Teneurin‑2 at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Teneurin-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Teneurin-2
  Met1-Lys183
  Accession # AAI72353
• Specificity: Detects Teneurin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Teneurin-3 is observed, and less than 4% cross-reactivity with rhTeneurin-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TENM2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Teneurin-2 Antibody [Unconjugated]

• teneurin-2
• ODZ2
• Ten-2
• Tenascin-M2
• teneurin transmembrane protein 2
• Teneurin2
• Teneurin-2
• Ten-m2
• TNM2

Background

Teneurin-2 (also Ten-2/Ten-m2, tenascin-M2, and Ten-m/Odz2) is a 250-330 kDa member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar Purkinje cells, pyramidal neurons of the hippocampus, and neurons of layers II and VI of the cerebral cortex. Teneurin-2 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human teneurin-2 is a 2774 amino acid (aa) type II TM glycoprotein. It contains a 379 aa cytoplasmic region (aa 1-379) that contains a polySer segment (aa 331-334), plus a 2374 aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa 575-841), five NHL repeats, each of which forms a beta -propeller (aa 1272-1573), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. There is a furin cleavage site after Arg528. There are three potential splice variants. One shows a deletion of aa 799-807, while two others show 46 and five aa substitutions for aa 1-167, respectively. Over aa 1-183, human teneurin-2 shares 98% aa identity with mouse teneurin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Teneurin-2 Antibody (AF4578)(2)

Publications using AF4578

• NV Vysokov, JP Silva, VG Lelianova, J Suckling, J Cassidy, JK Blackburn, N Yankova, MB Djamgoz, SV Kozlov, AG Tonevitsky, YA Ushkaryov Proteolytically released Lasso/teneurin-2 induces axonal attraction by interacting with latrophilin-1 on axonal growth cones Elife, 2018-11-20;7(0):. 2018-11-20 [PMID: 30457553] (Western Blot, Rat)
• Nickolai V. Vysokov, John-Paul Silva, Vera G. Lelianova, Claudia Ho, Mustafa B. Djamgoz, Alexander G. Tonevitsky et al. The Mechanism of Regulated Release of Lasso/Teneurin-2 Frontiers in Molecular Neuroscience 7/22/2016 [PMID: 27499734]

Bioinformatics

• Gene Symbol: TENM2
• Uniprot:
  • AAI72353()