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TEM8/ANTXR1 Antibody (200C1339(SB20)) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
200C1339(SB20)
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56585AF350
Alexa Fluor 405
NB100-56585AF405
Alexa Fluor 488
NB100-56585AF488
Alexa Fluor 532
NB100-56585AF532
Alexa Fluor 594
NB100-56585AF594
Alexa Fluor 647
NB100-56585AF647
Alexa Fluor 700
NB100-56585AF700
Alexa Fluor 750
NB100-56585AF750
Biotin
NB100-56585B
DyLight 350
NB100-56585UV
DyLight 405
NB100-56585V
DyLight 488
NB100-56585G
DyLight 550
NB100-56585R
DyLight 594
NB100-56585DL594
DyLight 650
NB100-56585C
DyLight 680
NB100-56585FR
DyLight 755
NB100-56585IR
HRP
NB100-56585H
Janelia Fluor 549
NBP2-33232JF549
Janelia Fluor 646
NBP2-33232JF646
Unconjugated
NB100-56585
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56585
Azide Free
NBP2-33232
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TEM8/ANTXR1 Antibody (200C1339(SB20)) [FITC] Summary

Immunogen
This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
Specificity
TEM8 is observed at ~80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ANTXR1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TEM8/ANTXR1 Antibody (200C1339(SB20)) [FITC]

  • anthrax toxin receptor 1
  • ANTXR1
  • ATR2310008J16Rik
  • FLJ10601
  • FLJ11298
  • FLJ21776
  • TEM8
  • TEM82810405N18Rik
  • Tumor endothelial marker 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56585AF350
Alexa Fluor 405 NB100-56585AF405
Alexa Fluor 488 NB100-56585AF488
Alexa Fluor 532 NB100-56585AF532
Alexa Fluor 594 NB100-56585AF594
Alexa Fluor 647 NB100-56585AF647
Alexa Fluor 700 NB100-56585AF700
Alexa Fluor 750 NB100-56585AF750
Biotin NB100-56585B
DyLight 350 NB100-56585UV
DyLight 405 NB100-56585V
DyLight 488 NB100-56585G
DyLight 550 NB100-56585R
DyLight 594 NB100-56585DL594
DyLight 650 NB100-56585C
DyLight 680 NB100-56585FR
DyLight 755 NB100-56585IR
FITC NB100-56585F
HRP NB100-56585H
Janelia Fluor 549 NBP2-33232JF549
Janelia Fluor 646 NBP2-33232JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ANTXR1