TEM7/PLXDC1 Antibody

Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line cancer tissue, HeLa human cervical epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TEM7/PLXDC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5327) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TEM7 Isoform 1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TEM7/PLXDC1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TEM7/PLXDC1 Isoform 1
  Leu19-Thr426
  Accession # ACE86780
• Specificity: Detects human TEM7/PLXDC1 Isoform 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TEM8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PLXDC1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TEM7/PLXDC1 Antibody

• FLJ45632
• plexin domain containing 1,2410003I07Rik
• plexin domain-containing protein 1
• PLXDC1
• TEM3
• TEM3FLJ36270
• TEM7
• TEM7DKFZp686F0937
• Tumor endothelial marker 3
• Tumor endothelial marker 7

Background

TEM7 (Tumor endothelial marker 7; also TEM3 and Plexin domain-containing protein-1/PXDC1) is an 85 kDa member of the plexin family of proteins. It is one of a number of TEM designated molecules that are related only a name and not structural similarity. TEM7 is found on tumor endothelial cells, newly formed endothelial cells and neurons. It is known to bind nidogen. Mature human TEM7 is a type I transmembrane glycoprotein 482 amino acids (aa) in length. It contains an extracellular region (aa 19‑426) with one plexin domain (aa 303‑347). There are three isoform variants. One is intracellular and shows an alternate start site at Met74 accompanied by a 28 aa substitution for aa 331‑500. The other two are soluble, with one showing only the above 28 aa substitution and a second showing an eight aa substitution for aa 331‑500. Over aa 19‑426, human TEM7 shares 81% aa identity with mouse TEM7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLXDC1
• Uniprot:
  • ACE86780()