TEM5/GPR124 Antibody - BSA Free

Western Blot: TEM5/GPR124 Antibody [NBP1-77315] - Human bladder tissue lysate with GPR124 antibody at (A) 2 and (B) 4 ug/ml.

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody [NBP1-77315] - Human bladder tissue with GPR124 antibody at 5 ug/ml.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TEM5/GPR124 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human TEM5. The immunogen is located within amino acids 1270 - 1320 of TEM5. Amino Acid Squence: CGALEKESHRRSYPLN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADGRA2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-4 ug/ml
• Control Peptide:

  TEM5/GPR124 Peptide (NBP1-77315PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TEM5/GPR124 Antibody - BSA Free

• DKFZp434C211
• DKFZp434J0911
• G protein-coupled receptor 124
• GPR124
• G-protein coupled receptor 124
• KIAA1531
• KIAA1531FLJ14390
• TEM5
• TEM5Tumor endothelial marker 5

Background

Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADGRA2
• Uniprot:
  • Q96PE1()