GPR125 Antibody

Images

 
Western Blot: GPR125 Antibody [NBP1-03561] - (0.5ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: GPR125 Antibody [NBP1-03561] - Bouin's fixed Human Testis stained with anti-GPR125

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GPR125 Antibody Summary

Immunogen
Peptide with sequence CTNKSASSFKNS corresponding to internal region according to NP_660333.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPR125
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.5 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 140 kDa band observed in human ovary lysates (calculated MW of 146 kDa band according to NP_660333.2). An additional band of unknown identity was also consistently observed at 33 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: An anonymous customer has reported successful results on human testis.
Theoretical MW
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-03561 in the following applications:

Publications
Read Publications using NBP1-03561.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPR125 Antibody

  • FLJ38547
  • G protein-coupled receptor 125
  • GPR125
  • PGR21
  • probable G-protein coupled receptor 125
  • TEM5L

Background

GPR125 is an orphan receptor which has a leucine rich repeat (LRR), an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2067
Species: Hu, Po, Ca
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NBP1-85747
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P, IP
NB600-1160
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF7977
Species: Hu, Mu, Rt
Applications: WB, IHC
NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NLS1720
Species: Hu
Applications: IHC-P
AF4634
Species: Hu
Applications: WB, Flow, CyTOF-ready
NLS2167
Species: Hu, Po, Bt, Ca, Eq, Mk, Rb
Applications: IHC-P
NLS418
Species: Hu
Applications: IHC-P
AF4894
Species: Hu
Applications: WB, Neut
PPS010
Species: Hu, Mu, Rt, Bv, Ch, Xp, Ze
Applications: WB
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
MAB8496
Species: Hu
Applications: Flow, CyTOF-ready
NBP2-45720
Species: Hu
Applications: WB, IHC, IF
NLS1513
Species: Hu
Applications: IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-89700
Species: Hu
Applications: IHC, IHC-P

Publications for GPR125 Antibody (NBP1-03561)(2)

Review for GPR125 Antibody (NBP1-03561) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP1-03561:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GPR125 NBP1-03561
Enlarge
reviewed by:
Marco Seandel
IHC-P Human 05/06/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTestis
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GPR125 Antibody (NBP1-03561) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GPR125 Products

Bioinformatics Tool for GPR125 Antibody (NBP1-03561)

Discover related pathways, diseases and genes to GPR125 Antibody (NBP1-03561). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GPR125 Antibody (NBP1-03561)

Discover more about diseases related to GPR125 Antibody (NBP1-03561).
 

Pathways for GPR125 Antibody (NBP1-03561)

View related products by pathway.

PTMs for GPR125 Antibody (NBP1-03561)

Learn more about PTMs related to GPR125 Antibody (NBP1-03561).

Blogs on GPR125

There are no specific blogs for GPR125, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Marco Seandel
05/06/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol GPR125
Entrez
Uniprot