TEM5/GPR124 Antibody - BSA Free

Images

 
Western Blot: TEM5/GPR124 Antibody [NBP1-76844] - Rat kidney tissue lysate with GPR124 antibody at (A) 2 ug/ml and (B) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: TEM5/GPR124 Antibody [NBP1-76844] - in human bladder tissue
Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody [NBP1-76844] - Human bladder tissue with GPR124 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

TEM5/GPR124 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the center of human TEM5. The immunogen is located within amino acids 560 - 610 of TEM5. Amino Acid Squence: PPEPEPPADQQLR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADGRA2
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2 - 4 ug/ml
Control Peptide
TEM5/GPR124 Peptide (NBP1-76844PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for TEM5/GPR124 Antibody - BSA Free

  • DKFZp434C211
  • DKFZp434J0911
  • G protein-coupled receptor 124
  • GPR124
  • G-protein coupled receptor 124
  • KIAA1531
  • KIAA1531FLJ14390
  • TEM5
  • TEM5Tumor endothelial marker 5

Background

Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TEM5/GPR124 Antibody (NBP1-76844) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ADGRA2
Entrez
Uniprot