TCR alpha Antibody

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Western Blot: TCR alpha Antibody [NBP1-45883] - Analysis of TCRa expression in Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TCR alpha Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 138 of human TCR&alpha.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRA
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 4 ug/mL
  • Immunohistochemistry 15 - 25 ug/mL
  • Immunoprecipitation 10 - 20 ug/mL

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 30% glycerol, 0.5% BSA, 5 mM EDTA
Preservative
0.03% Proclin
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for TCR alpha Antibody

  • C)
  • D
  • FLJ22602
  • J
  • MGC117436
  • MGC22624
  • MGC23964
  • MGC71411
  • T cell receptor alpha locus
  • T-cell antigen receptor, alpha polypeptide
  • T-cell receptor, alpha (V
  • TCRA
  • TCRD
  • TRA

Background

The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on TCR alpha.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

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Bioinformatics

Gene Symbol TRA
Entrez