KALRN Antibody

Immunohistochemistry-Paraffin: KALRN Antibody [NB100-41371] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

Flow Cytometry: KALRN Antibody [NB100-41371] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: KALRN Antibody [NB100-41371] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Ma, Mu, Rt
• Applications: ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KALRN Antibody Summary

• Description: Novus Biologicals Goat KALRN Antibody (NB100-41371) is a polyclonal antibody validated for use in IHC, ELISA and Flow. Anti-KALRN Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RWHLGPGDPFSTYV, from the C Terminus of the protein sequence according to NP_003938.1; NP_001309918.1; NP_001309919.1; NP_001309920.1; NP_001309921.1.
• Specificity: This antibody is expected to recognise NP_003938.1 (isoform 2), NP_001309918.1 (isoform 5), NP_001309919.1 (isoform 6), NP_001309920.1 (isoform 7), and NP_001309921.1 (isoform 8).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KALRN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 4 - 5 ug/mL
  • Immunohistochemistry-Paraffin 4 - 5 ug/mL
  • Peptide ELISA Detection Limit 1:16000

Reactivity Notes

Mammal reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KALRN Antibody

• ARHGEF24
• DUETFLJ16443
• DUO
• EC 2.7.11.1
• FLJ12332
• FLJ18623
• HAPIP
• huntingtin-associated protein interacting protein (duo)
• Huntingtin-associated protein-interacting protein
• kalirin
• kalirin, RhoGEF kinase
• Protein Duo
• serine/threonine kinase with Dbl- and pleckstrin homology domains
• Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain
• TRADFLJ18196

Background

The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KALRN Antibody (NB100-41371)(2)

Publications using NB100-41371

• Wosczyna MN, Konishi CT, Perez Carbajal EE et al. Reciprocal Activation within a Kinase-Effector Complex Underlying Persistence of Structural LTP Neuron 2019-05-08 [PMID: 31078368]
• Colomer V, Engelender S, Sharp AH et al. Huntingtin-associated protein 1 (HAP1) binds to a Trio-like polypeptide, with a rac1 guanine nucleotide exchange factor domain. Hum Mol Genet 1997-09-01 [PMID: 9285789]

Review for KALRN Antibody (NB100-41371) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer ELISA Other 05/12/2010

Summary

• Application: ELISA
• Sample Tested: NB100-41371
• Species: Other

Bioinformatics

• Gene Symbol: KALRN
• Entrez:
  • 8997(Human)
  • 545156(Mouse)
  • 84009(Rat)
• Uniprot:
  • O60229(Human)