TCL1A Antibody

Western Blot: TCL1 Antibody [NB100-77332] - (0.1ug/ml) staining of Jurkat Cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TCL1A Antibody Summary

• Immunogen: Peptide with sequence C-QLYPDGRYRSSD corresponding to internal region according to NP_068801.1.
• Epitope: QLYPDGRYRSSD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TCL1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 16 kDa band observed in Daudi and Jurkat lysates (calculated MW of 13.5 kDa band according to NP_068801.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TCL1A Antibody

• Oncogene TCL1
• Oncogene TCL-1
• Protein p14 TCL1
• T-cell leukemia/lymphoma 1A
• T-cell lymphoma-1
• T-cell lymphoma-1A
• TCL1A
• TCL1-PEN
• TCL1T-cell leukemia/lymphoma protein 1A

Background

The T-cell leukaemia/lymphoma 1 (TCL1)-family oncoproteins augment AKT signal transduction and enhance cell proliferation and survival. Chromosome rearrangements, faulty developmental silencing and Epstein-Barr virus infection appear to dysregulate the expression of TCL1-family genes, provoking several important types of lymphocyte cancer. A key role for TCL1 proteins in cell transformation has been established in studies of transgenic mouse models, which develop a unique spectrum of T- and B-cell malignancies. How TCL1 proteins are regulated and dysregulated, how they promote transformation and the potential for therapies modelled on TCL1 interactions have important implications for understanding and treating lymphocyte cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCL1A Antibody (NB100-77332)(1)

Publication using NB100-77332

• Shen RR, Ferguson DO, Renard M et al. Dysregulated TCL1 requires the germinal center and genome instability for mature B-cell transformation. Blood 2006-09-15 [PMID: 16728701]

Bioinformatics

• Gene Symbol: TCL1A
• Entrez:
  • 8115(Human)
• Uniprot:
  • NP_068801.1(Human)