TCF7/TCF1 Antibody (812145) [Unconjugated]

Western blot shows lysates of EL-4 mouse lymphoblast cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TCF7/TCF1 at approximately 30-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 812145
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TCF7/TCF1 Antibody (812145) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse TCF7/TCF1
  Gly61-Gly116
  Accession # Q00417
• Specificity: Detects mouse TCF7/TCF1 in ELISA and Western Blot.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TCF7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF7/TCF1 Antibody (812145) [Unconjugated]

• FLJ36364
• MGC47735
• T-cell factor 1
• T-cell specific
• TCF1
• TCF7
• TCF-7
• transcription factor 7 (T-cell specific, HMG-box)

Background

TCF7 (Transcription factor 7), also called TCF1 (T cell factor 1) is a member of the lymphoid enhancer binding factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within aa 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF7/TCF1 Antibody (MAB8224)(4)

Publications using MAB8224

• Avishai Gavish, Benny Chain, Tomer M. Salame, Yaron E. Antebi, Shir Nevo, Shlomit Reich-Zeliger et al. From pseudo to real-time dynamics of T cell thymic differentiation iScience 2023-01-20 [PMID: 36624839]
• Qiuyu Guo, Albert Kim, Bin Li, Andrew Ransick, Helena Bugacov, Xi Chen et al. A beta -catenin-driven switch in TCF/LEF transcription factor binding to DNA target sites promotes commitment of mammalian nephron progenitor cells eLife 2021-02-15 [PMID: 33587034]
• Qiuyu Guo, Albert Kim, Bin Li, Andrew Ransick, Helena Bugacov, Xi Chen et al. A beta -catenin-driven switch in TCF/LEF transcription factor binding to DNA target sites promotes commitment of mammalian nephron progenitor cells eLife 2021-02-15 [PMID: 33587034] (Immunocytochemistry, Mouse)
• Jina Ko, Yongcheng Wang, Jonathan C. T. Carlson, Angela Marquard, Jeremy Gungabeesoon, Alain Charest et al. Single Extracellular Vesicle Protein Analysis Using Immuno‐Droplet Digital Polymerase Chain Reaction Amplification Advanced Biosystems 2020-12-01 [PMID: 33274611]

Bioinformatics

• Gene Symbol: TCF7
• Uniprot:
  • Q00417()