TCF7/TCF1 Antibody (7H3)

Western Blot: TCF7/TCF1 Antibody (7H3) [NBP2-29766] - Jurkat cell lysate probed with anti-TCF-1 at 1ug/ml.

Product Discontinued

TCF7/TCF1 Antibody (7H3) Summary

• Immunogen: Maltose binding protein (MBP) fusion protein corresponding to residues 102-269 of human TCF-1A splice variant. The epitope maps to a region just N-terminal to the High Mobility Group (HMG) box.
• Specificity: Recognizes multiple splice isoforms of TCF-1.
  26kDa,32kDa,38kDa,42kDa,and48kDa weight may be detected.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.2M Tris-Glycine (pH 7.5) and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.84 mg/ml
• Purity: Protein G purified

Notes

Relevant Entrez Gene Numbers: NM_003202

Alternate Names for TCF7/TCF1 Antibody (7H3)

• FLJ36364
• MGC47735
• T-cell factor 1
• T-cell specific
• TCF1
• TCF7
• TCF-7
• transcription factor 7 (T-cell specific, HMG-box)

Background

TCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with b catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor a enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with b catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCF7
• Uniprot:
  • P36402(Human)