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TCF-3/E2A Antibody

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Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NB100-57092] - TCF3 / E2A Antibody (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NB100-57092] - TCF3 / E2A Antibody (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
SDS-Page: TCF-3/E2A Antibody [NB100-57092] - Analysis of TCF3/E2A in Daudi cell lysate (35ug protein in RIPA buffer) using this antibody at 0.01ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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TCF-3/E2A Antibody Summary

Immunogen
Peptide with sequence C-KAPRARTSPDEDED corresponding to internal region according to NP_003191.1.
Epitope
KAPRARTSPDEDED
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TCF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 75 kDa band observed in Daudi lysates (calculated MW of 67.6 kDa band according to NP_003191.1). IHC-P: Human kidney shows strong nuclear staining in DCT cells.
Control
TCF-3/E2A Overexpression Lysate
Publications
Read Publications using
NB100-57092 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TCF-3/E2A Antibody

  • bHLHb21
  • bHLHb21TCF-3
  • Class B basic helix-loop-helix protein 21
  • E2A immunoglobulin enhancer-binding factor E12/E47
  • E2A
  • E2AKappa-E2-binding factor
  • Immunoglobulin enhancer-binding factor E12/E47
  • Immunoglobulin transcription factor 1
  • ITF1
  • ITF1Transcription factor ITF-1
  • Kappa-E2-binding factor
  • MGC129647
  • MGC129648
  • Pan2
  • TCF-3
  • Tcfe2a
  • transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
  • Transcription factor 3
  • transcription factor E2-alpha
  • VDIR
  • VDR interacting repressor

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TCF-3/E2A Antibody (NB100-57092)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Control Lysate(s)

TCF-3/E2A Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TCF3
Entrez
Uniprot