TCF-3/E2A Antibody

Immunohistochemistry-Paraffin: TCF-3/E2A Antibody [NB100-57092] - TCF3 / E2A Antibody (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

SDS-Page: TCF-3/E2A Antibody [NB100-57092] - Analysis of TCF3/E2A in Daudi cell lysate (35ug protein in RIPA buffer) using this antibody at 0.01ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TCF-3/E2A Antibody Summary

• Description: Novus Biologicals Goat TCF-3/E2A Antibody (NB100-57092) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-TCF-3/E2A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KAPRARTSPDEDED corresponding to internal region according to NP_003191.1.
• Epitope: KAPRARTSPDEDED
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TCF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in Daudi lysates (calculated MW of 67.6 kDa band according to NP_003191.1). IHC-P: Human kidney shows strong nuclear staining in DCT cells.
• Control:

  TCF-3/E2A Overexpression Lysate

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28402854).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TCF-3/E2A Antibody

• bHLHb21
• bHLHb21TCF-3
• Class B basic helix-loop-helix protein 21
• E2A immunoglobulin enhancer-binding factor E12/E47
• E2A
• E2AKappa-E2-binding factor
• Immunoglobulin enhancer-binding factor E12/E47
• Immunoglobulin transcription factor 1
• ITF1
• ITF1Transcription factor ITF-1
• Kappa-E2-binding factor
• MGC129647
• MGC129648
• Pan2
• TCF-3
• Tcfe2a
• transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Transcription factor 3
• transcription factor E2-alpha
• VDIR
• VDR interacting repressor

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF-3/E2A Antibody (NB100-57092)(2)

Publications using NB100-57092

• Walters B J, Coak E et al. In Vivo Interplay between p27Kip1, GATA3, ATOH1, and POU4F3 Converts Non-sensory Cells to Hair Cells in Adult Mice. Cell Rep 2017-11-04 [PMID: 28402854] (IF/IHC, Mouse)
• Greenbaum S, Lazorchak AS, Zhuang Y. Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. J Biol Chem 2004-10-22 [PMID: 15310760]

Bioinformatics

• Gene Symbol: TCF3
• Entrez:
  • 6929(Human)
• Uniprot:
  • NP_003191.1(Human)