TCF-3/E2A Antibody (401703) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 401703
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

TCF-3/E2A Antibody (401703) [Alexa Fluor® 647] Summary

• Immunogen: E.coli-derived recombinant human TCF-3/E2A
  Gln100-Glu500
  Accession # NP_003191
• Specificity: Detects human TCF-3/E2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TCF-1 (aa 116‑233), rhTCF-1 (aa 217-358), rhTCF-1 alpha/LEF-1, rhTCF-2, rhTCF-4, rhTCF-12, rhTCF7L1 (aa 151-320), rhTCFL1 (a
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF-3/E2A Antibody (401703) [Alexa Fluor® 647]

• bHLHb21
• bHLHb21TCF-3
• Class B basic helix-loop-helix protein 21
• E2A immunoglobulin enhancer-binding factor E12/E47
• E2A
• E2AKappa-E2-binding factor
• Immunoglobulin enhancer-binding factor E12/E47
• Immunoglobulin transcription factor 1
• ITF1
• ITF1Transcription factor ITF-1
• Kappa-E2-binding factor
• MGC129647
• MGC129648
• Pan2
• TCF-3
• Tcfe2a
• transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Transcription factor 3
• transcription factor E2-alpha
• VDIR
• VDR interacting repressor

Background

TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.