TCF-2/HNF-1 beta Antibody [Unconjugated]

TCF-2/HNF-1 beta was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

TCF-2/HNF-1 beta was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

TCF-2/HNF-1 beta was detected in perfusion fixed frozen sections of rat kidney using Goat Anti-Human TCF-2/HNF-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3330) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TCF-2/HNF-1 beta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TCF‑2/HNF‑1 beta
  Gly51-Gln182
  Accession # P35680
• Specificity: This antibody detects TCF‑2/HNF‑1 beta in direct ELISAs and Western blots. It is expected to recognize all three TCF‑2 isoforms.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HNF1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF-2/HNF-1 beta Antibody [Unconjugated]

• FJHN
• hepatocyte nuclear factor 1-beta
• HNF1 beta A
• HNF-1 beta
• HNF1 homeobox B
• HNF1B
• HNF-1B
• HNF1beta
• HNF-1-beta
• HNF2
• Homeoprotein LFB3
• LFB3
• LF-B3
• MODY5
• MODY5HPC11
• TCF2
• TCF-2
• TCF2transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor
• Transcription factor 2
• transcription factor 2, hepatic
• Variant hepatic nuclear factor 1
• VHNF1

Background

TCF-2, also known as HNF-1 beta (hepatocyte nuclear factor 1 beta ) and VHNF-1, is a member of the HNF-1 homeobox family. By alternative splicing, three isoforms of TCF-2/HNF-1 beta exist. TCF-2/HNF-1 beta binds DNA as a homodimer or heterodimer with TCF-1/HNF-1 alpha . Within the region used as the immunogen, human TCF-2 shares 98% amino acid sequence homology with mouse and rat TCF-2. TCF-2 expression is required for visceral endoderm specification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF-2/HNF-1 beta Antibody (AF3330)(3)

Publications using AF3330

• Vethe H, Bjorlykke Y, Ghila LM et al. Probing the missing mature B-cell proteomic landscape in differentiating patient iPSC-derived cells Sci Rep 2017-07-06 [PMID: 28684784]
• Yuki Ohi, Han Qin, Chibo Hong, Laure Blouin, Jose M. Polo, Tingxia Guo et al. Incomplete DNA methylation underlies a transcriptional memory of the somatic cell in human iPS cells Nature Cell Biology 2011-05-01 [PMID: 21499256]
• Yuki Ohi, Han Qin, Chibo Hong, Laure Blouin, Jose M. Polo, Tingxia Guo et al. Incomplete DNA methylation underlies a transcriptional memory of the somatic cell in human iPS cells Nature Cell Biology 2011-05-01 [PMID: 21499256]

Bioinformatics

• Gene Symbol: HNF1B
• Uniprot:
  • P35680()