TC-PTP/PTPN2 Antibody - BSA Free

Western Blot: TC-PTP/PTPN2 Antibody [NBP2-48528] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4

Immunocytochemistry/Immunofluorescence: TC-PTP/PTPN2 Antibody [NBP2-48528] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human kidney.

Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human colon, kidney, lymph node and testis using Anti-PTPN2 antibody NBP2-48528 (A) shows similar protein distribution across tissues to independent antibody NBP2-48841 (B).

Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human testis.

Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human colon.

Immunohistochemistry-Paraffin: TC-PTP/PTPN2 Antibody [NBP2-48528] - Staining of human lymph node.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TC-PTP/PTPN2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: DDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSESALRKRIREDRKATTAQKVQQMKQRLNENERK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TC-PTP/PTPN2 Recombinant Protein Antigen (NBP2-48528PEP)

Reactivity Notes

Mouse (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TC-PTP/PTPN2 Antibody - BSA Free

• protein tyrosine phosphatase, non-receptor type 2
• PTP2
• PTPN2
• PTPTPTN2
• T-cell protein tyrosine phosphatase
• T-cell protein-tyrosine phosphatase
• TCELLPTP
• TCPTP
• TC-PTP
• TCPTPEC 3.1.3.48
• tyrosine-protein phosphatase non-receptor type 2

Background

PTPN2 is encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Three alternatively spliced variants of this gene, which encode isoforms differing at their extreme C-termini, have been described. The different C-termini are thought to determine the substrate specificity, as well as the cellular localization of the isoforms. Two highly related but distinctly processed pseudogenes that localize to distinct chromosomes have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPN2