TC-PTP/PTPN2 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TC-PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TC-PTP at approximately 45 to 48 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for TC‑PTP at approximately 51 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat TC‑PTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1930) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TC-PTP/PTPN2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TC-PTP
  Pro2-Asn314
  Accession # P17706
• Specificity: Detects human, mouse and rat TC-PTP in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TC-PTP/PTPN2 Antibody [Unconjugated]

• protein tyrosine phosphatase, non-receptor type 2
• PTP2
• PTPN2
• PTPTPTN2
• T-cell protein tyrosine phosphatase
• T-cell protein-tyrosine phosphatase
• TCELLPTP
• TCPTP
• TC-PTP
• TCPTPEC 3.1.3.48
• tyrosine-protein phosphatase non-receptor type 2

Background

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kDa isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kDa isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52^Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TC-PTP/PTPN2 Antibody (AF1930)(2)

Publications using AF1930

• Jason P. Twohig, Ana Cardus Cardus Figueras, Robert Andrews, Florian Wiede, Benjamin C. Cossins, Alicia Derrac Derrac Soria et al. Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells Nature Immunology 4/1/2019 [PMID: 30890796]
• Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A Integrin alpha1beta1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. J. Biol. Chem., 2010-10-12;285(51):40114-24. 2010-10-12 [PMID: 20940300] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PTPN2
• Uniprot:
  • P17706()