TC-PTP/PTPN2 Antibody [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Mu, Rt
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 594

TC-PTP/PTPN2 Antibody [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human, mouse and rat TC-PTP in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TC-PTP/PTPN2 Antibody [Alexa Fluor™ Plus 594]

• protein tyrosine phosphatase, non-receptor type 2
• PTP2
• PTPN2
• PTPTPTN2
• T-cell protein tyrosine phosphatase
• T-cell protein-tyrosine phosphatase
• TCELLPTP
• TCPTP
• TC-PTP
• TCPTPEC 3.1.3.48
• tyrosine-protein phosphatase non-receptor type 2

Background

T-cell protein tyrosine phosphatase (TC-PTP), also known as PTPT and PTPN2, is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. This enzyme has two C-terminal end splice variants with distinctly different subcellular localizations. The shorter 45 kDa isoform is exclusively nuclear in resting cells, but redistrubutes to the cytosol upon stimulation with growth factors (1) and cellular stress (2). The longer 48 kDa isoform is exclusively found in the endoplasmic reticulum (3) and seems to have distinctly different physiologic substrates from the smaller isoform (1, 4). Although found in many cell types and tissues, TC-PTP is particularly prominent in hemopoietic cell types (5, 6). Knockout mice lacking TC-PTP are born viable but die 3 to 5 weeks after birth of erythropoietic and lymphopoietic deficits (7), indicating a critical role for TC-PTP in bone marrow maturation. TC-PTP will dephosphorylate a wide range of phosphoproteins, such as p52^Shc (6) and receptors for EGF (1), Insulin (8) and growth hormone (6). The recombinant protein lacks the C-terminal 100 amino acids that determine intracellular localization but is fully active (9).

1. Tiganis, T. et al. (1999) J. Biol. Chem. 274:27768.
2. Lam, M.H. et al. (2001) J. Biol. Chem. 276:37700.
3. Lorenzen, J.A. et al. (1995) J. Cell Biol. 131:631.
4. Tiganis, T. et al. (1998) Mol. Cell. Biol. 18:1622.
5. Cool, D.E. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5257.
6. Pasquali, C. et al. (2003) Mol. Endocrinol. 17:2228.
7. You-Ten, K.E. et al. (1997) J. Exp. Med. 186:683.
8. Galic, S. et al. (2003) Mol. Cell. Biol. 23:2096.
9. Cool, D.E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7280.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.