TBX6 Antibody [Unconjugated]

Western blot shows lysates of HT1080 human fibrosarcoma cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TBX6 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

TBX6 was detected in immersion fixed frozen sections of embryonic mouse mesoderm (E9.5) using 10 µg/mL Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

TBX6 was detected in immersion fixed JOY6 human induced pluripotent stem cells undifferentiated (right panel) and differentiated into mesoderm (left panel) using Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) at 0.2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei in mesoderm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Generation of NMPs from EpiSCs.(A) Brachyury/Sox2 immunocytochemistry in EpiSC cultures treated with FGF/CHIR for 72 h. (B) qPCR analysis for indicated markers in mouse EpiSCs treated with FGF/CHIR. Error bars = s.d. (n = 3). n/d, not determined. Results are represented as log10 ratio of expression versus untreated EpiSCs. The data used to generate the plot can be found in Data S4. (C) Combined fluorescence/brightfield microscopy showing donor cell incorporation of grafted GFP+ EpiSC differentiated for 48 h in FGF/CHIR after 48 h embryo culture. (D) Table summarizing the incorporation of grafted GFP+ EpiSC differentiated for 24 h or 48 h in Fgf/Wnt within host embryos. NT, neural tube; Som, somite; PSM, presomitic mesoderm; n/a, not applicable. (E) Representative examples of donor cell incorporation (green, GFP) and differentiation (red, immunofluorescence for indicated markers). Cell nuclei were stained with DAPI (blue). White boxes indicate the position of magnified images of GFP+ cells. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25157815), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TBX6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TBX6
  Met1-Arg280
  Accession # O95947
• Specificity: Detects human TBX6 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TBX6
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 0.2-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TBX6 Antibody [Unconjugated]

• DFNB67
• T-box 6
• T-box protein 6
• T-box transcription factor TBX6
• TBX6

Background

TBX6 (T-box protein 6) is a 47 kDa member of the T-box family of transcriptional regulators. It is expressed during embryogenesis, plays a role in presomitic mesoderm development, and promotes delta-like 1 transcription. Human TBX6 is 436 amino acids (aa) in length. It contains one T‑box DNA-binding domain (aa 90‑277). There is one alternate splice form that shows a 15 aa substitution for aa 281‑436. Over aa 1‑280, human TBX6 shows 94% and 90% aa identity with canine and mouse TBX6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TBX6 Antibody (AF4744)(20)

Publications using AF4744

• Atash, A;Cramer, MJ;Mees, B;Doevendans, PA;Sluijter, JPG;Stillitano, F; Generation and characterization of novel induced pluripotent stem cell (iPSC) lines derived from three symptomatic carriers of a pathogenic MYH11 variant and two non-carrier relatives Stem cell research 2024-12-12 [PMID: 39705883] (Immunocytochemistry, Human)
• Daya, NM;Döring, K;Zhuge, H;Volke, L;Stab, V;Dietz, J;Athamneh, M;Roos, A;Zaehres, H;Güttsches, AK;Mavrommatis, L;Vorgerd, M; Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C Stem cell research 2024-01-27 [PMID: 38309149] (Immunocytochemistry, Human)
• Boeing, A;Mavrommatis, L;Daya, NM;Zhuge, H;Volke, L;Kocabas, A;Kneifel, M;Athamneh, M;Krause, K;Südkamp, N;Döring, K;Theiss, C;Roos, A;Zaehres, H;Güttsches, AK;Vorgerd, M; Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease Stem cell research 2023-10-04 [PMID: 37839261] (ICC, Human)
• Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F; Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative Stem cell research 2023-09-21 [PMID: 37748331] (ICC, Human)
• Marina Gattiglio, Michelle Protzek, Christian Schröter Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells Biology Open 2023-08-15 [PMID: 37530863]
• NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau A gastruloid model of the interaction between embryonic and extra-embryonic cell types Journal of tissue engineering, 2022-06-11;13(0):2041731422110. 2022-06-11 [PMID: 35707767] (IHC, Mouse)
• MK Shin, JS Bang, JE Lee, HD Tran, G Park, DR Lee, J Jo Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration International Journal of Molecular Sciences, 2022-05-04;23(9):. 2022-05-04 [PMID: 35563499] (IHC, Human)
• Meritxell Sáez, Robert Blassberg, Elena Camacho-Aguilar, Eric D. Siggia, David A. Rand, James Briscoe Statistically derived geometrical landscapes capture principles of decision-making dynamics during cell fate transitions Cell Systems 2022-01-19 [PMID: 34536382] (Flow Cytometry, Immunocytochemistry, Mouse)
• Oh, S, Song, H Et al. Cooperation between ETS transcription factor ETV1 and histone demethylase JMJD1A in colorectal cancer. Int J Oncol 12/1/2020 [PMID: 33174020]
• André Dias, Anastasiia Lozovska, Filip J Wymeersch, Ana Nóvoa, Anahi Binagui-Casas, Daniel Sobral et al. A Tgfbr1/Snai1-dependent developmental module at the core of vertebrate axial elongation eLife 2020-06-29 [PMID: 32597756]
• AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020-04-07;31(1):107471. 2020-04-07 [PMID: 32268087] (ICC, Human)
• Robert J. Garriock, Ravindra B. Chalamalasetty, JianJian Zhu, Mark W. Kennedy, Amit Kumar, Susan Mackem et al. A dorsal-ventral gradient of Wnt3a/ beta -catenin signals controls mouse hindgut extension and colon formation Development 2020-01-01 [PMID: 32156757]
• M Chen, M Maimaitili, SH Buchholdt, UB Jensen, F Febbraro, M Denham Generation of eight human induced pluripotent stem cell lines from Parkinson's disease patients carrying familial mutations Stem Cell Res, 2019-11-13;42(0):101657. 2019-11-13 [PMID: 31786474] (ICC, Human)
• Li-Fang Chu, Daniel Mamott, Zijian Ni, Rhonda Bacher, Cathy Liu, Scott Swanson et al. An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells Cell Reports 2019-08-27 [PMID: 31461642]
• Aida Rodrigo Rodrigo Albors, Pamela A. Halley, Kate G. Storey Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions Development 2018-10-01 [PMID: 30201686]
• Thomas JR Frith, Ilaria Granata, Matthew Wind, Erin Stout, Oliver Thompson, Katrin Neumann et al. Human axial progenitors generate trunk neural crest cells in vitro eLife 2018-08-10 [PMID: 30095409]
• Anestis Tsakiridis, Valerie Wilson Assessing the bipotency of in vitro-derived neuromesodermal progenitors F1000Research 2015-07-31 [PMID: 26401264]
• Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation. Development, 2015-05-01;142(9):1628-38. 2015-05-01 [PMID: 25922526] (IHC-Fr, Mouse)
• Gouti , Mina, Tsakiridis , Anestis, Wymeersch , Filip J, Huang , Yali, Kleinjung , Jens, Wilson , Valerie, Briscoe , James In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol, 2014-08-26;12(8):e1001937. 2014-08-26 [PMID: 25157815] (IHC, Human)
• Cheung C, Bernardo AS, Trotter MW Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility. Nat. Biotechnol., 2012-01-15;30(2):165-73. 2012-01-15 [PMID: 22252507] (ICC, Human)

Bioinformatics

• Gene Symbol: TBX6
• Uniprot:
  • O95947()