TBX2 Antibody (7G5)

Western Blot: TBX2 Antibody (7G5) [H00006909-M01] - TBX2 monoclonal antibody (M01), clone 7G5 Analysis of TBX2 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: TBX2 Antibody (7G5) [H00006909-M01] - Analysis of monoclonal antibody to TBX2 on HeLa cell. Antibody concentration 30 ug/ml.

Western Blot: TBX2 Antibody (7G5) [H00006909-M01] - Analysis of TBX2 over-expressed 293 cell line, cotransfected with TBX2 Validated Chimera RNAi ( Cat # H00006909-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TBX2 monoclonal antibody (M01), clone 7G5 (Cat # H00006909-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: TBX2 Antibody (7G5) [H00006909-M01] - Analysis of TBX2 expression in transfected 293T cell line by TBX2 monoclonal antibody (M01), clone 7G5.Lane 1: TBX2 transfected lysate(74.1 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: TBX2 Antibody (7G5) [H00006909-M01] - Detection limit for recombinant GST tagged TBX2 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, S-ELISA, KD
• Clone: 7G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TBX2 Antibody (7G5) Summary

• Immunogen: TBX2 (NP_005985, 603 a.a. ~ 702 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GSARPRLRFSPYQIPVTIPPSTSLLTTGLASEGSKAAGGNSREPSPLPELALRKVGAPSRGALSPSGSAKEAANELQSIQRLVSGLESQRALSPGRESPK
• Specificity: TBX2 - T-box 2
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBX2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28035050).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TBX2 Antibody (7G5)

• FLJ10169
• T-box 2
• T-box protein 2
• T-box transcription factor TBX2
• TBX2

Background

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product is the human homolog of mouse Tbx2, and shares strong sequence similarity with Drosophila omb protein. Expression studies indicate that this gene may have a potential role in tumorigenesis as an immortalizing agent. Transcript heterogeneity due to alternative polyadenylation has been noted for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TBX2 Antibody (H00006909-M01)(1)

Publication using H00006909-M01

• Fantini D , Huang S, Asara JM et al. Chromatin Association of XRCC5/6 in the Absence of DNA Damage Depends upon the XPE Gene Product DDB2. Molecular Biology of The Cell. Nov 9 2016 [PMID: 28035050] (WB, Mouse)

Bioinformatics

• Gene Symbol: TBX2
• Entrez:
  • 6909(Human)
• OMIM:
  • 600747(Human)
• Uniprot:
  • NP_005985(Human)