TBK1 Antibody [Unconjugated]

TBK1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for TBK1 at approximately 90 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

• Reactivity: Hu
• Applications: Simple Western, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

TBK1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TBK-1
  Met1-Gly121
  Accession # Q9UHD2
• Specificity: Detects human TBK-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 20 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TBK1 Antibody [Unconjugated]

• EC 2.7.11
• EC 2.7.11.1
• FLJ11330
• FTDALS4
• NAK serine/threonine-protein kinase TBK 1
• NAK
• NAKserine/threonine-protein kinase TBK1
• NF-kappa-B-activating kinase
• NFKB-Activating Kinase
• T2K
• TANK-binding kinase 1NF-kB-activating kinase
• TBK 1
• TBK1

Background

TANK binding kinase-1 (TBK1), encoded by the TBK1 gene, is a multimeric Serine/threonine-protein kinase that mediates a crosstalk between the innate immune response and autophagy. TBK1 was first identified as a TANK-interacting protein controlling NF-kappa B-mediated responses. Mutations in TBK1 have been found in conditions like amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), normal tension glaucoma (NTG) and childhood herpes simplex encephalitis (HSE). Gain-of-function of TBK1 are associated with NTG, whereas loss-of-function mutations result in ALS/FTD or in HSE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TBK1 Antibody (AF9934). (Showing 1 - 6 of 6 FAQ).

1. What is the exact sequence this TBK1 antibody recognizes?
   • The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
2. I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
   • While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
3. We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
   • Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
4. Is there a smaller size of your TBK1 antibody?
   • This TBK1 antibody is offered in a smaller 0.025 mg size.
5. Are there any publications where this TBK1 antibody was used in western blot for mouse?
   • This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
6. Cant this TBK1 antibody be conjugated to PE for FLOW?
   • Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

Bioinformatics

• Uniprot:
  • Q9UHD2()