TBC1D4 Antibody

Images

 
Western Blot: TBC1D4 Antibody [NB100-57094] - (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TBC1D4 Antibody [NB100-57094] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TBC1D4 Antibody Summary

Immunogen
Peptide with sequence C-DDPEKIEERKKSK corresponding to internal region according to NP_055647.2.
Epitope
DDPEKIEERKKSK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TBC1D4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 150 kDa band observed in lysates of Daudi and MOLT4 (calculated MW of 147 kDa band according to NP_055647.2). IHC-P: Human skeletal muscle shows striated pattern staining of majority of muscular fibres.
Publications
Read Publications using NB100-57094.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TBC1D4 Antibody

  • Akt substrate of 160 kDa
  • AS160Acrg embryonic lethality minimal region ortholog
  • DKFZp779C0666
  • KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein
  • TBC1 domain family member 4
  • TBC1 domain family, member 4

Background

AS160 is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of threonine 642 is also important in mediating AS160 redistribution from low density microsomes to the cytosol in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TBC1D4 Antibody (NB100-57094)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBC1D4
Entrez
Uniprot