TAZ/WWTR1 Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

TAZ/WWTR1 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Synthetic peptide representing residues 386-400 of the human TAZ protein.
• Specificity: Reacts with residues 386-400, which is the PDZ-binding domain of the 49kDa human TAZ protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WWTR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human. Mouse reactivity reported in scientific literature (PMID: 28065575)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TAZ/WWTR1 Antibody [CoraFluor™ 1]

• FLJ27004
• FLJ45718
• TAZ
• TAZDKFZp586I1419
• transcriptional co-activator with PDZ-binding motif
• Transcriptional coactivator with PDZ-binding motif
• WW domain containing transcription regulator 1
• WW domain-containing transcription regulator protein 1
• WWTR1

Background

TAZ is a YAP-related protein that contains a WW domain and transcriptional activation region. TAZ plays a role in mesenchymal stem cell differentiation. The antibody was designed in such a way that should generate a TAZ specific-reagent able to differentiate between YAP and TAZ proteins in cells and tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TAZ/WWTR1 Antibody (NB600-220CL1). (Showing 1 - 1 of 1 FAQ).

1. I want to know which TAZ primary antibody is best produced for WB (human and mouse)!
   • I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.

Bioinformatics

• Gene Symbol: WWTR1