Tau [p Ser262] Antibody

Images

 
Western Blot: Tau [p Ser262] Antibody [NB100-82242] - Analysis of extracts from mouse brain tissue using Tau(Phospho-Ser262) Antibody and the same antibody preincubated with blocking peptide.
Immunocytochemistry/ Immunofluorescence: Tau [p Ser262] Antibody [NB100-82242] - Staining of methanol-fixed Hela cells using Tau(Phospho-Ser262) antibody
Immunohistochemistry: Tau [p Ser262] Antibody [NB100-82242] - Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using Tau (phospho-Ser262) antibody.
Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Tau [p Ser262] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 262 (I-G-SP-T-E).
Modification
p Ser262
Marker
pan-Neuronal Marker
Specificity
Tau (specific to Phospho-Ser262) detects endogenous levels of Tau only when phosphorylated at serine 262.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Simple Western
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Positive Control
Tau Lysate (NBL1-12894)
Reviewed Applications
Read 1 Review rated 3
using
NB100-82242 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tau [p Ser262] Antibody

  • DDPAC
  • FLJ31424
  • FTDP-17
  • G protein beta1/gamma2 subunit-interacting factor 1
  • MAPT
  • MGC138549
  • microtubule-associated protein tau
  • MSTD
  • MSTDMAPTL
  • MTBT1
  • MTBT1Neurofibrillary tangle protein
  • MTBT2
  • Neurofibrillary tangle protein
  • PHF-tau
  • PPND
  • Tau
  • TAUPaired helical filament-tau

Background

Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tau Antibody (NB100-82242) (0)

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Review for Tau Antibody (NB100-82242) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Simple Western.

Reviews using NB100-82242:
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Simple Western
(1)
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Images Ratings Applications Species Date Details
Simple Western Tau NB100-82242
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reviewed by:
Anonymous
Simple Western Mouse 08/05/2015
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Summary

ApplicationSimple Western
SpeciesMouse
Special ApplicationsImage courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tau Antibody (NB100-82242) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Tau Antibody (NB100-82242)

Discover related pathways, diseases and genes to Tau Antibody (NB100-82242). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Tau Antibody (NB100-82242)

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Blogs on Tau.

The C99 fragment of amyloid precursor protein (APP)
Alzheimer’s Disease (AD) is a neurodegenerative disorder that is characterized by an abundance of the beta-amyloid peptide in the brain.  When AD was first discovered, it was determined that beta-amyloid was produced as a result of the prote...  Read full blog post.

Tau - A microtubule associated protein as a biomarker for Alzheimer's disease
The tau protein is a microtubule associated protein found mostly in neuronal cells where it regulates the stability of axonal microtubules as well as kinesin-dependent transport. Tau is relevant in the study of various neurological disorders as ab...  Read full blog post.

PINK1: All work and no fun
The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and...  Read full blog post.

Using Amyloid beta peptides in Alzheimer's Disease Immunization
Amyloid beta (AB) peptide has a central role in the neurodegeneration of Alzheimer's disease (AD). Immunization of AD transgenic mice with AB-42 peptide reduces both the spatial memory impairments and AD-like neuropathologic changes.Therapeutic im...  Read full blog post.

New Study Links Tau Mutations to Microglial Immune Response
Tau proteins are abundant in the axons of neurons in the central nervous system (CNS), and play a key role in microtubule formation and stabilization. Antibody studies have identified six tau isoforms, all produced by alternative mRNA splicing of the...  Read full blog post.

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Recent Reviews

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Anonymous
08/05/2015
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol MAPT
Entrez
Uniprot