Tau [p Ser262] Antibody

Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.

Western Blot: Tau [p Ser262] Antibody [NB100-82242] - Western blot analysis of lysates from HeLa cells, using NB100-82242. The lane on the left is blocked with the phospho peptide.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Tau [p Ser262] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of serine 262 (I-G-SP-T-E).
• Modification: p Ser262
• Marker: pan-Neuronal Marker
• Specificity: Detects endogenous levels of Tau only when phosphorylated at serine 262.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western
  • Western Blot 1:500-1:2000
• Control:

  Tau Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tau [p Ser262] Antibody

• DDPAC
• FLJ31424
• FTDP-17
• G protein beta1/gamma2 subunit-interacting factor 1
• MAPT
• MGC138549
• microtubule-associated protein tau
• MSTD
• MSTDMAPTL
• MTBT1
• MTBT1Neurofibrillary tangle protein
• MTBT2
• Neurofibrillary tangle protein
• PHF-tau
• PPND
• Tau
• TAUPaired helical filament-tau

Background

Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Tau Antibody (NB100-82242) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Simple Western Mouse 08/05/2015

Summary

• Application: Simple Western
• Species: Mouse

Bioinformatics

• Gene Symbol: MAPT
• Entrez:
  • 4137(Human)
  • 17762(Mouse)
  • 29477(Rat)
• Uniprot:
  • P10636()