Talin1 Antibody (8D4)

Product Discontinued

Talin1 Antibody (8D4) Summary

• Immunogen: Cytoskeletal protein talin, purified from chicken gizzard.
• Localization: Cell Membrane and Intracllular
• Specificity: Monoclonal anti-Talin recognizes an epitope located on the intact talin molecule (225 kDa) and the 190 kDa fragment but not on the 47 kDa fragment obtained by protease cleavage of chicken gizzard talin, when used in immunoblotting.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLN1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: immunofluorescence and Western Blot: In WB expected bands at 225 kDa and 190 kDa. The antibody also stains a slightly higher molecular weight talin (235 kDa) of mammalian platelets.The antibody stains focal adhesions, the membrane ruffles, and ventral streaks in cultured fibroblasts of mammalian (human, rat), avian (chicken) and amphibian (Xenopus), but not fish, in indirect immunofluorescence. NB120-11188 may also be used microinjection and transfection studies. Optimal dilutions and concentrations should be determined by the end user.
• Theoretical MW: 230 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Frog. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Raw ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Talin1 Antibody (8D4)

• ILWEQ
• KIAA1027
• talin 1
• Talin1
• talin-1
• TLN
• TLN1

Background

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Talin 2 is related to talin 1. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Talin1 Antibody (NB600-1029)(2)

Publications using NB600-1029

• Ostrowski PP, Freeman SA, Fairn G, Grinstein S Dynamic Podosome-Like Structures in Nascent Phagosomes Are Coordinated by Phosphoinositides Dev. Cell 2019-06-06 [PMID: 31231039]
• Ostrowski P Macrophage Surveillance and Immunity: Eating, Drinking, and Digesting Thesis 2020-01-01 (ICC/IF, Human)

FAQs for Talin1 Antibody (NB600-1029). (Showing 1 - 1 of 1 FAQs).

1. I bought Talin antibody (8d4) for western blot and it looks like this product is acites, not purified antibody. What kind of blocking method do you recommend, BSA, or milk, or something else?
   • This antibody is unpurified ascites. We recommend using this antibody in western blot with 5% nonfat dry milk in TBST (0.1% Tween-20). We will use this buffer for blocking (1-2 hours room temperature) and for the antibody diluent.

Bioinformatics

• Gene Symbol: TLN1
• Entrez:
  • 395194(Chicken)
  • 7094(Human)
• Uniprot:
  • P54939(Chicken)