TACE/ADAM17 Antibody

Western Blot: TACE/ADAM17 Antibody [NB100-1012] - Staining of Human HeLa lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 0.2 ug/mL. Detected by chemiluminescence.

Product Discontinued

TACE/ADAM17 Antibody Summary

• Immunogen: Peptide with sequence LQRQNRVDSKETEC corresponding to C-Terminus according to NP_003174.3.
• Localization: Type I membrane protein.
• Specificity: This antibody is expected to recognize isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAM17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 1 ug/mL
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TACE/ADAM17 Antibody

• ADAM 17
• ADAM metallopeptidase domain 17
• ADAM metallopeptidase domain 18
• ADAM17
• ADAM18
• CD156b antigen
• CD156b
• CSVP
• disintegrin and metalloproteinase domain-containing protein 17
• EC 3.4.24.86
• MGC71942
• Snake venom-like protease
• TACE
• TACEcSVP
• TNF-alpha convertase
• TNF-alpha converting enzyme
• TNF-alpha-converting enzyme
• tumor necrosis factor, alpha, converting enzyme

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACE/ADAM17 Antibody (NB100-1012)(1)

Publication using NB100-1012

• Black RA, Rauch CT, Kozlosky CJ et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature 1997-02-20 [PMID: 9034190]

Bioinformatics

• Gene Symbol: ADAM17
• Entrez:
  • 6868(Human)
  • 11491(Mouse)
• Uniprot:
  • NP_003174.3.(Human)