TACE/ADAM17 Antibody

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACE/ADAM17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9301) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TACE/ADAM17 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TACE/ADAM17 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human TACE/ADAM17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9301) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TACE/ADAM17 Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TACE/ADAM17
  Arg215-Asn671
  Accession # P78536
• Specificity: Detects human TACE/ADAM17 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAM17
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TACE/ADAM17 Antibody

• ADAM 17
• ADAM metallopeptidase domain 17
• ADAM metallopeptidase domain 18
• ADAM17
• ADAM18
• CD156b antigen
• CD156b
• CSVP
• disintegrin and metalloproteinase domain-containing protein 17
• EC 3.4.24.86
• MGC71942
• Snake venom-like protease
• TACE
• TACEcSVP
• TNF-alpha convertase
• TNF-alpha converting enzyme
• TNF-alpha-converting enzyme
• tumor necrosis factor, alpha, converting enzyme

Background

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha , TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha , Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACE/ADAM17 Antibody (AF9301)(4)

Publications using AF9301

• Gerrit Hugendieck, Marcus Lettau, Svenja Andreas, Sabrina Neumann, Natalie Reinhardt, Philipp Arnold et al. Chemotherapy‐induced release of ADAM17 bearing EV as a potential resistance mechanism in ovarian cancer Journal of Extracellular Vesicles 2023-07-05 [PMID: 37408115]
• Dylan Frabutt, Natalie Stull, Annie R. Pineros, Sarah A. Tersey, Donalyn Scheuner, Teresa L. Mastracci et al. Adiponectin receptor fragmentation in mouse models of type 1 and type 2 diabetes Archives of Autoimmune Diseases 2021-05-28 [PMID: 34414399]
• I González-F, P Santulli, S Chouzenoux, F Carmona, C Chapron, F Batteux Dysregulation of the ADAM17/Notch signalling pathways in endometriosis: from oxidative stress to fibrosis Mol. Hum. Reprod., 2017-07-01;0(0):1-12. 2017-07-01 [PMID: 28486700] (Western Blot, Human)
• Shao , Shuhong, Li , Zunling, Gao , Wei, Yu , Guohua, Liu , Dexiang, Pan , Fang ADAM-12 as a diagnostic marker for the proliferation, migration and invasion in patients with small cell lung cancer. PLoS ONE, 2014-01-21;9(1):e85936. 2014-01-21 [PMID: 24465799] (IHC-P, Human)

Bioinformatics

• Gene Symbol: ADAM17
• Uniprot:
  • P78536()