TACE/ADAM17 Antibody (1F6)

Western Blot: TACE/ADAM17 Antibody (1F6) [H00006868-M01] - ADAM17 monoclonal antibody (M01), clone 1F6. Analysis of ADAM17 expression in human spleen.

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody (1F6) [H00006868-M01] - Analysis of monoclonal antibody to ADAM17 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

Proximity Ligation Assay: TACE/ADAM17 Antibody (1F6) [H00006868-M01] - Analysis of protein-protein interactions between TGFA and ADAM17. HeLa cells were stained with anti-TGFA rabbit purified polyclonal 1:1200 and anti-ADAM17 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: TACE/ADAM17 Antibody (1F6) [H00006868-M01] - Detection limit for recombinant GST tagged ADAM17 is approximately 3ng/ml as a capture antibody.

Product Discontinued

TACE/ADAM17 Antibody (1F6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ADAM17 (NP_003174, 215 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RADPDPMKNTCKLLVVADHRFYRYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGIQIEQIRILKSPQEVKPGEKHYNMAKSYPNEEKDAWDV
• Specificity: ADAM17 - a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM17
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein for WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TACE/ADAM17 Antibody (1F6)

• ADAM 17
• ADAM metallopeptidase domain 17
• ADAM metallopeptidase domain 18
• ADAM17
• ADAM18
• CD156b antigen
• CD156b
• CSVP
• disintegrin and metalloproteinase domain-containing protein 17
• EC 3.4.24.86
• MGC71942
• Snake venom-like protease
• TACE
• TACEcSVP
• TNF-alpha convertase
• TNF-alpha converting enzyme
• TNF-alpha-converting enzyme
• tumor necrosis factor, alpha, converting enzyme

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TACE/ADAM17 Antibody (H00006868-M01)(1)

Publication using H00006868-M01

• Kahlert C, Weber H, Mogler C et al. Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer 2009-08-04 [PMID: 19603023]

Bioinformatics

• Gene Symbol: ADAM17
• Entrez:
  • 6868(Human)
• OMIM:
  • 603639(Human)
• Uniprot:
  • NP_003174(Human)