T-bet/TBX21 Antibody (525803) [Unconjugated]

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol." > MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol." title="Jurkat human acute T cell leukemia cell line was stained with Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol." />

Jurkat human acute T cell leukemia cell line was stained with Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # <a class=

HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." > HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." title="Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line . PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for T-bet/TBX21 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." />

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line . PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

MAB002) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). Cells were then stained with Mouse Anti-Human CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1509A) and Mouse Anti-Human CD45RO PE‑conjugated Monoclonal Antibody (Catalog # FAB10642P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." > MAB002) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). Cells were then stained with Mouse Anti-Human CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1509A) and Mouse Anti-Human CD45RO PE‑conjugated Monoclonal Antibody (Catalog # FAB10642P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." title="CD45RO+/CD8+ PBMC lymphocytes were stained with either (A) Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) or (B) isotype control antibody (Catalog # MAB002) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). Cells were then stained with Mouse Anti-Human CD8 alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB1509A) and Mouse Anti-Human CD45RO PE‑conjugated Monoclonal Antibody (Catalog # FAB10642P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." />

CD45RO+/CD8+ PBMC lymphocytes were stained with either (A) Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) or (B) isotype control antibody (Catalog # <a class=

Product Discontinued

T-bet/TBX21 Antibody (525803) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human T-bet
  Glu326-Asn535
  Accession # Q9UL17
• Specificity: Detects human T-bet.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBX21
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for T-bet/TBX21 Antibody (525803) [Unconjugated]

• Tbet
• T-bet
• TBETT-PET
• TBLYM
• TBLYMT-cell-specific T-box transcription factor T-bet
• T-box 21
• T-box expressed in T cells
• T-box protein 21
• T-box transcription factor TBX21
• TBX21
• T-PET
• Transcription factor TBLYM

Background

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma , and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for T-bet/TBX21 Antibody (MAB5385)(4)

Publications using MAB5385

• Hu Q, Liao P, Li W et al. Clinical Use of Propranolol Reduces Biomarkers of Proliferation in Gastric Cancer Frontiers in Oncology 4/26/2021 [PMID: 33981600]
• Hendriks A, van der Velden H, Wolberink E, Seyger M, Schalkwijk J, Zeeuwen P, de Jong E, Pasch M, van Erp P, van de Kerkhof P The effect of adalimumab on key drivers in the pathogenesis of psoriasis. Br J Dermatol, 2014-03-01;170(3):571-80. 2014-03-01 [PMID: 24640989] (IHC, Human)
• Nyirenda MH, Sanvito L, Darlington PJ TLR2 Stimulation Drives Human Naive and Effector Regulatory T Cells into a Th17-Like Phenotype with Reduced Suppressive Function. J. Immunol., 2011-07-20;187(5):2278-90. 2011-07-20 [PMID: 21775683] (Flow Cytometry, Human)
• Mansfield AS, Holtan SG, Grotz TE Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis. Mod. Pathol., 2010-12-10;24(4):487-94. 2010-12-10 [PMID: 21151098] (IHC-P, Human)

FAQs for T-bet/TBX21 Antibody (MAB5385). (Showing 1 - 1 of 1 FAQs).

1. Do you make a Tbx21 ab for FACS for porcine?
   • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Bioinformatics

• Gene Symbol: TBX21
• Uniprot:
  • Q9UL17(Human)