Syntaxin-BP1 [p Ser241] Antibody - Azide Free

Western Blot: STXBP1 [p Ser241] Antibody [NBP2-29507] - Western blot of mouse hippocampus lysate showing specific immunolabeling of the ~65k Munc18-1 protein phosphorylated at Ser241. Phosphospecificity is shown in the second lane where immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).

• Reactivity: Mu, Rt, Hu, Rt, Bv, Ca, Ch, Pm, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Syntaxin-BP1 [p Ser241] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser241 conjugated to KLH. Accession # O08599
• Modification: p Ser241
• Specificity: Specific for endogenous levels of the ~65 kDa Syntaxin-BP1 protein phosphorylated at Ser241. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Human (100%), Rat (100%), Chicken (100%), Canine (100%), Sheep (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STXBP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500
  • Western Blot 1:1000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Syntaxin-BP1 [p Ser241] Antibody - Azide Free

• EIEE4
• FLJ37475
• hUNC18
• MUNC18-1
• NSEC1
• N-Sec1
• p67
• Protein unc-18 homolog 1
• Protein unc-18 homolog A
• rbSec1
• STXBP1
• syntaxin binding protein 1
• syntaxin-binding protein 1
• SyntaxinBP1
• Syntaxin-BP1
• UNC18
• unc18-1
• UNC18A
• Unc-18A
• UNC18neuronal SEC1

Background

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc18-1 is thought to be regulated by phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STXBP1
• Entrez:
  • 20910(Mouse)
• Uniprot:
  • O08599(Mouse)