Syntaxin 6 Antibody - BSA Free

Western Blot: Syntaxin 6 Antibody [NBP2-15132] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 6 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Syntaxin 6 Antibody [NBP2-15132] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntaxin 6 protein stained by Syntaxin 6 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Syntaxin 6 Antibody [NBP2-15132] - Paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.

Western Blot: Syntaxin 6 Antibody [NBP2-15132] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Syntaxin 6 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Syntaxin 6. The exact sequence is proprietary.
• Marker: TGN Marker
• Predicted Species: Porcine (97%), Bovine (97%), Rhesus Macaque (99%), Chicken (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STX6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Syntaxin 6 Antibody - BSA Free

• STX6
• Syntaxin 6
• syntaxin-6

Background

Involved in intracellular vesicle trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: STX6
• Uniprot:
  • O43752(Human)