Use1/UBE2Z Antibody

Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Analysis in human cell line RPMI-8226.

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Western Blot: Use1/UBE2Z Antibody [NBP1-82785] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human lymphoid node shows moderate cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP1-82785] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Use1/UBE2Z Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RSELLGTDSAEPEMDVRKRTGVAGSQPVSEKQSAAELDLVLQRHQNLQEKLAEEMLGLARSLKTNTLAAQSVIKKDNQTLSHSLKMADQNLEKLKTESERLEQHTQKSVNW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:100-1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Use1/UBE2Z Protein (NBP1-82785PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Use1/UBE2Z Antibody

• MDS032
• p31
• protein p31
• putative MAPK activating protein PM26
• Putative MAPK-activating protein PM26
• Q-SNARE
• SLT1
• SNARE-like tail-anchored protein 1 homolog
• UBE2Z
• unconventional SNARE in the ER 1 homolog (S. cerevisiae)
• Use1
• USE1L
• USE1-like protein
• vesicle transport protein USE1

Background

SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USE1