Syndecan-2/CD362 Antibody (305515R) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
305515R
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 647

Syndecan-2/CD362 Antibody (305515R) [Alexa Fluor® 647] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-2/CD362
Glu19-Gly144
Accession # AAH49836
Specificity
Detects human Syndecan-2/CD362 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Syndecan-1, recombinant human (rh) Syndecan-3, or rhSyndecan-4 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syndecan-2/CD362 Antibody (305515R) [Alexa Fluor® 647]

  • CD362 antigen
  • CD362
  • Fibroglycan
  • heparan sulfate proteoglycan 1, cell surface-associated
  • Heparan sulfate proteoglycan core protein
  • HSPG
  • HSPG1
  • HSPG1syndecan proteoglycan 2
  • SDC2
  • SYND2
  • SYND2cell surface-associated heparan sulfate proteoglycan 1
  • syndecan 2
  • Syndecan2
  • Syndecan-2

Background

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2 is dependent on the transmembrane domain (5). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines (6). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGFbasic, VEGF and EGF (7). Syndecan-2 expressed on human primary osteoblasts binds GM-CSF and may function as a co‑receptor (8). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (9). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (10).

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.
  4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.
  5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
  6. Park, H. et al. (2002) J. Biol. Chem. 277:29730.
  7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.
  8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.
  9. Halden, Y. et al. (2004) Biochem. J. 377:533.
  10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB29651U
Alexa Fluor 405 FAB29651V
Alexa Fluor 488 FAB29651G
Alexa Fluor 594 FAB29651T
Alexa Fluor 647 FAB29651R
Alexa Fluor 700 FAB29651N
Alexa Fluor 750 FAB29651S

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