Syndecan-1/CD138 Antibody (359103R) [Unconjugated]

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RPMI 8226 human multiple myeloma cell line was stained with Rat Anti-Human Syndecan‑1/CD138 Monoclonal Antibody (Catalog # MAB2780R, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
359103R
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

Syndecan-1/CD138 Antibody (359103R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
Gln18-Glu251
Accession # NP_002988
Specificity
Detects human Syndecan-1/VD138 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syndecan-1/CD138 Antibody (359103R) [Unconjugated]

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the syndecan family of Type 1 transmembrane proteins (1, 2). The four syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 17 aa signal sequence, a 234 aa extracellular domain (ECD) that includes three closely-spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 25 aa TM segment, and a 34 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site. The ECD is variably modified by GAGs, producing molecular weights of 120-200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Human Syndecan-1 ECD shares 65-71% aa identity with the ECD of rat, mouse, canine, equine and bovine Syndecan-1. Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (4, 5). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (4-8). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (5, 7, 9). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (10-12). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (12, 13). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (4).

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
  3. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  4. Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
  5. Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
  6. Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
  7. Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
  8. Vanhoutte, D. et al. (2007) Circulation 115:475.
  9. Li, Q. et al. (2002) Cell 111:635.
  10. Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
  11. Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
  12. Derksen, P.W.B. et al. (2002) Blood 99:1405.
  13. Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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