Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Mouse SYT1 in samples. No significant cross-reactivity or interference between Mouse SYT1 and analogues was observed. |
Standard Curve Range |
0.156-10 ng/mL |
Sensitivity |
0.094 ng/mL |
Assay Type |
Sandwich-ELISA |
Inter-Assay |
CV% < 4.7% |
Intra-Assay |
CV% < 4.85% |
Spike Recovery |
90-106% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
SYT1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25X)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Mouse Synaptotagmin 1 ELISA Kit (Colorimetric)
Background
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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