Mouse Synaptotagmin 1 ELISA Kit (Colorimetric)

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ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Samples were spiked with high concentrations of Mouse Synaptotagmin 1 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL

Order Details

Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse SYT1 in samples. No significant cross-reactivity or interference between Mouse SYT1 and analogues was observed.
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.094 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.7%
Intra-Assay
CV% < 4.85%
Spike Recovery
90-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
SYT1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25X)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Synaptotagmin 1 ELISA Kit (Colorimetric)

  • DKFZp781D2042
  • P65
  • SVP65
  • synaptotagmin Isynaptotagmin 1
  • Synaptotagmin1
  • Synaptotagmin-1
  • SYT
  • SYT1
  • sytI

Background

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SYT1
Uniprot