Synaptotagmin-1 Antibody

Images

 
Western Blot: Synaptotagmin 1 Antibody [NBP1-91499] - Synaptotagmin 1 in bovine oocytes. Image from confirmed customer review.
Immunocytochemistry/ Immunofluorescence: Synaptotagmin 1 Antibody [NBP1-91499] - Sample Type: outer mouse plexiform layer, Red: Primary, Blue: DAPI, Primary Dilution: 1:200, Secondary Antibody: Goat anti-Rabbit AF568 ...read more
Western Blot: Synaptotagmin 1 Antibody [NBP1-91499] - Mouse Spleen Lysate 1ug/ml Gel Concentration 12%
Immunocytochemistry/ Immunofluorescence: Synaptotagmin 1 Antibody [NBP1-91499] - complete mouse retina sections Red: Primary Blue: DAPI Primary Dilution: 1:200 Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L) ...read more

Product Details

Summary
Reactivity Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Synaptotagmin-1 Antibody Summary

Immunogen
Synthetic peptide directed towards the middle region of human Syt1. Peptide sequence FDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against Syt1 and was validated on Western blot.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-91499 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Synaptotagmin-1 Antibody

  • DKFZp781D2042
  • P65
  • SVP65
  • synaptotagmin Isynaptotagmin 1
  • Synaptotagmin1
  • Synaptotagmin-1
  • SYT
  • SYT1
  • SytI

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Synaptotagmin-1 Antibody (NBP1-91499) (0)

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Review for Synaptotagmin-1 Antibody (NBP1-91499) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-91499:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Synaptotagmin-1 NBP1-91499
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reviewed by:
Allison Tscherner
WB Other 02/20/2014
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Summary

ApplicationWestern Blot
Sample TestedOocytes
SpeciesOther
LotQC30410

Blocking

Blocking Details60min 5% milk in TBST at room temp

Primary Anitbody

Dilution Ratio1:1000 at 4 degrees C overnight

Secondary Antibody

Secondary DescriptionAnti-rabbit IgG, HRP
Secondary Manufacturer Cat#CST#7074
Secondary Concentration1:2000

Details

Detection NotesECL

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Synaptotagmin-1 Antibody (NBP1-91499) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Synaptotagmin-1 Antibody (NBP1-91499)

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Diseases for Synaptotagmin-1 Antibody (NBP1-91499)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Allison Tscherner
02/20/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol SYT1
Entrez
Uniprot