Synaptotagmin-1 Antibody

Images

 
Western Blot: Synaptotagmin-1 Antibody [NBP1-51925] - (1ug/ml) Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Synaptotagmin-1 Antibody [NBP1-51925] - NBP1-51925 (3.8ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Synaptotagmin-1 Antibody Summary

Immunogen
Peptide with sequence C-RDLQSAEKEEQEK corresponding to internal region according to NP_005630.1.
Epitope
C-RDLQSAEKEEQEK
Specificity
Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SYT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 65 kDa band observed in human brain is reported in scientific literature (PMID: 7298720). IHC-P: Human brain shows vesicular staining in neuronal cell and axon.
Publications
Read Publication using NBP1-51925.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat. Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Synaptotagmin-1 Antibody

  • DKFZp781D2042
  • P65
  • SVP65
  • synaptotagmin Isynaptotagmin 1
  • Synaptotagmin1
  • Synaptotagmin-1
  • SYT
  • SYT1
  • sytI

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
DDX0131P-100
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-88650
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-01345
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NB100-56507
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2699
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NB100-689
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP2-47415
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF3235
Species: Hu, Mu, Rt
Applications: WB, IHC
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC

Publications for Synaptotagmin-1 Antibody (NBP1-51925)(1)


Showing Publication 1 - 1 of 1.
Publication using NBP1-51925 Applications Species
Martens S, Kozlov MM, McMahon HT. How synaptotagmin promotes membrane fusion. Science 2007 May 25 [PMID: 17478680]

Reviews for Synaptotagmin-1 Antibody (NBP1-51925) (0)

There are no reviews for Synaptotagmin-1 Antibody (NBP1-51925). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Synaptotagmin-1 Antibody (NBP1-51925) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Synaptotagmin-1 Products

Bioinformatics Tool for Synaptotagmin-1 Antibody (NBP1-51925)

Discover related pathways, diseases and genes to Synaptotagmin-1 Antibody (NBP1-51925). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Synaptotagmin-1 Antibody (NBP1-51925)

Discover more about diseases related to Synaptotagmin-1 Antibody (NBP1-51925).
 

Pathways for Synaptotagmin-1 Antibody (NBP1-51925)

View related products by pathway.

PTMs for Synaptotagmin-1 Antibody (NBP1-51925)

Learn more about PTMs related to Synaptotagmin-1 Antibody (NBP1-51925).
 

Research Areas for Synaptotagmin-1 Antibody (NBP1-51925)

Find related products by research area.

Blogs on Synaptotagmin-1

There are no specific blogs for Synaptotagmin-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Synaptotagmin-1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SYT1
Entrez
Uniprot