Synaptotagmin 1 Antibody

Western Blot: Synaptotagmin-1 Antibody [NBP1-51925] - (1ug/ml) Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Synaptotagmin-1 Antibody [NBP1-51925] - NBP1-51925 (3.8ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Discontinued

Synaptotagmin 1 Antibody Summary

• Immunogen: Peptide with sequence C-RDLQSAEKEEQEK corresponding to internal region according to NP_005630.1.
• Epitope: C-RDLQSAEKEEQEK
• Specificity: Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SYT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in human brain is reported in scientific literature (PMID: 7298720). IHC-P: Human brain shows vesicular staining in neuronal cell and axon.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Synaptotagmin 1 Antibody

• DKFZp781D2042
• P65
• SVP65
• synaptotagmin Isynaptotagmin 1
• Synaptotagmin1
• Synaptotagmin-1
• SYT
• SYT1
• sytI

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first C2 domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synaptotagmin 1 Antibody (NBP1-51925)(1)

Publication using NBP1-51925

• Martens S, Kozlov MM, McMahon HT. How synaptotagmin promotes membrane fusion. Science 2007-05-25 [PMID: 17478680]

Bioinformatics

• Gene Symbol: SYT1
• Entrez:
  • 6857(Human)
  • 20979(Mouse)
  • 25716(Rat)
• Uniprot:
  • NP_005630.1(Human)