Symplekin Antibody - BSA Free

Western Blot: Symplekin Antibody [NB100-74591] - Detection of Human and Mouse Symplekin by Western Blot. Samples: Whole cell lysate from HeLa, (5, 15 and 50 ug), and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Symplekin antibody NB100-74591 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: Symplekin Antibody [NB100-74591] - Detection of human Symplekin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Symplekin antibody NB100-74591 (lot 2) used for IP at 6 ug per reaction. Symplekin was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-Symplekin antibody NB100-74590. For blotting immunoprecipitated Symplekin, NB100-74591 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Symplekin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Symplekin Antibody - BSA Free (NB100-74591) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human Symplekin using the numbering given in entry NP_004810.2 (GeneID 8189).
• Localization: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYMPK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 141 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Symplekin Antibody - BSA Free

• FLJ27092
• SPKSYM
• symplekin

Background

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYMPK
• Entrez:
  • 8189(Human)
• Uniprot:
  • Q92797(Human)