Symplekin Antibody

Images

 
Western Blot: Symplekin Antibody [NB100-74590] - Detection of Human Symplekin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more
Immunohistochemistry: Symplekin Antibody [NB100-74590] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Symplekin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

Symplekin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Symplekin using the numbering given in entry NP_004810.2 (GeneID 8189).
Localization
Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYMPK
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Symplekin Antibody

  • FLJ27092
  • SPKSYM
  • symplekin

Background

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SYMPK
Entrez
Uniprot