Symplekin Antibody - BSA Free

Immunohistochemistry: Symplekin Antibody [NB100-74590] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Symplekin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: Symplekin Antibody [NB100-74590] - Detection of human Symplekin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Symplekin antibody NB100-74590 (lot 2) used for IP at 6 ug per reaction. Symplekin was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated Symplekin, NB100-74590 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Symplekin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Symplekin Antibody - BSA Free (NB100-74590) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Symplekin using the numbering given in entry NP_004810.2 (GeneID 8189).
• Localization: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Nucleus, nucleoplasm. Note=Cytoplasmic face of adhesion plaques (major) and nucleoplasm (minor) (in cells with TJ). Nucleoplasm (in cells without
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYMPK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
• Application Notes: This antibody is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Symplekin Antibody - BSA Free

• FLJ27092
• SPKSYM
• symplekin

Background

This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYMPK
• Entrez:
  • 8189(Human)
• Uniprot:
  • NP_004810.2(Human)