Suppressor of Fused Antibody (HL3374)

Suppressor of Fused antibody [HL3374] detects Suppressor of Fused protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Suppressor of Fused stained by Suppressor of Fused antibody [HL3374] (NBP3-48798) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minCorresponding RNA expression data for the same tissues are based on NCBI.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3374] (NBP3-48798) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Suppressor of Fused antibody [HL3374] detects Suppressor of Fused protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.Suppressor of Fused stained by Suppressor of Fused antibody [HL3374] (NBP3-48798) diluted at 1:250.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3374] (NBP3-48798) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt
• Applications: WB
• Clone: HL3374
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Suppressor of Fused Antibody (HL3374) Summary

• Description: Novus Biologicals Rabbit Suppressor of Fused Antibody (HL3374) (NBP3-48798) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Suppressor of Fused.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SUFU
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Suppressor of Fused Antibody (HL3374)

• SUFUHSUFUXLPRO1280
• suppressor of fused homolog (Drosophila)
• suppressor of fused homolog

Background

The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused HSu(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). HSu(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUFU