SUMO3 Antibody [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line and mouse liver. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse SUMO3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2959) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SUMO3 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SUMO3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SUMO3
  Met1-Phe103
  Accession # P55854
• Specificity: Detects endogenous human and mouse proteins modified with SUMO3 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human SUMO2 and SUMO4 is observed, and less than 1% cross-reactivity with SUMO1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SUMO3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SUMO3 Antibody [Unconjugated]

• SMT3 (suppressor of mif two 3, yeast) homolog 1
• SMT3 homolog 1
• SMT3 suppressor of mif two 3 homolog 1
• SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 3 (yeast)
• SMT3A
• SMT3B
• SMT3H1
• SMT3H1small ubiquitin-related modifier 3
• SUMO-2
• SUMO3
• SUMO-3
• ubiquitin-like protein SMT3A
• Ubiquitin-like protein SMT3B

Background

Small Ubiquitin-like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. Unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All SUMO proteins share the conserved ubiquitin domain and the C‑terminal diglycine cleavage/attachment site. Human SUMO3, also known as SMT3A, is synthesized as a 103 amino acid (aa), 11.5 kDa propeptide that contains an 11 aa C‑terminal prosegment. Following prosegment cleavage, the C‑terminal glycine is enzymatically attached to a lysine on a target protein. Human SUMO3 shares 83% sequence identity with SUMO3 from mouse. SUMO3 also has very high sequence homology to SUMO2 and SUMO4, 87% and 75%, respectively. SUMO3 shares only 47% sequence identity to SUMO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUMO3
• Uniprot:
  • P55854()