Sulfatase Modifying Factor 1/SUMF1 Antibody (329005) [mFluor Violet 610 SE]

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clone: 329005
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

Sulfatase Modifying Factor 1/SUMF1 Antibody (329005) [mFluor Violet 610 SE] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Sulfatase Modifying Factor 1/SUMF1 isoform 1
  Ser34-Asp374 (Ser63Asn)
  Accession # Q8NBK3.3
• Specificity: Detects recombinant human or mouse Sulfatase Modifying Factor 1/SUMF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SUMF2 or recombinant mouse SUMF2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SUMF1
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Sulfatase Modifying Factor 1/SUMF1 Antibody (329005) [mFluor Violet 610 SE]

• AAPA3037
• EC 1.8.99.-
• FGE1
• FGEC-alpha-formylglycine-generating enzyme 1
• FGly-generating enzyme
• MGC131853
• MGC150436
• sulfatase modifying factor 1
• sulfatase-modifying factor 1
• SUMF1
• UNQ3037

Background

Sulfatases catalyze the hydrolysis of sulfate esters such as glycosaminoglycans, sulfolipids, and steroid sulfates. C-alpha-formylglycine (FGly), the catalytic residue in the active site of eukaryotic sulfatases, is posttranslationally generated from a cysteine by SUMF1, the FGly-generating enzyme (FGE), in the endoplasmic reticulum (ER). The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in multiple sulfatase deficiency (MSD; MIM 272200), a lysosomal storage disorder (Roeser et al., 2006 [PubMed 16368756]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUMF1