SUCLA2 Antibody - BSA Free

Western Blot: SUCLA2 Antibody [NBP2-94539] - Analysis of extracts of various cell lines, using SUCLA2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: SUCLA2 Antibody - BSA Free [NBP2-94539] - Immunofluorescence analysis of L929 using SUCLA2 antibody (A10040) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: SUCLA2 Antibody - BSA Free [NBP2-94539] - Immunofluorescence analysis of U2OS using SUCLA2 antibody (A10040) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SUCLA2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SUCLA2 Antibody - BSA Free (NBP2-94539) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1). MAASMFYGRLVAVATLRNHRPRTAQRAAAQVLGSSGLFNNHGLQVQQQQQRNLSLHEYMSMELLQEAGVSVPKGYVAKSPDEAYAIAKKLGSKDVVIKAQVLAGGRGKGTFESGLKGGVKIVFSPEEAKAVSSQMIGKKLFTKQTGEKGRICNQVLVCERKYPRREYYFAITMERSFQGP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SUCLA2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SUCLA2 Antibody - BSA Free

• A-BETA
• ATP-specific succinyl-CoA synthetase subunit beta
• beta subunit
• EC 6.2.1
• EC 6.2.1.5
• mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta
• MTDPS5
• Renal carcinoma antigen NY-REN-39
• succinate-CoA ligase, ADP-forming, beta subunit
• succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial

Background

Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUCLA2