SUCLG2 Antibody

Images

 
Western Blot: SUCLG2 Antibody [NBP1-32521] - Wild-type (WT) and SUCLG2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG2 antibody diluted at 1:500. ...read more
Immunocytochemistry/ Immunofluorescence: SUCLG2 Antibody [NBP1-32521] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SUCLG2 protein stained by SUCLG2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. ...read more
Immunohistochemistry-Paraffin: SUCLG2 Antibody [NBP1-32521] - Paraffin-embedded hepatoma. SUCLG2 antibody dilution: 1:500
Western Blot: SUCLG2 Antibody [NBP1-32521] - A: mouse liver 10% SDS PAGE diluted at 1:10000
Western Blot: SUCLG2 Antibody [NBP1-32521] - A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SUCLG2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SUCLG2. The exact sequence is proprietary.
Localization
Mitochondrion
Predicted Species
Rat (91%), Rhesus Macaque (98%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUCLG2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
SUCLG2 Knockout 293T Cell Lysate
Publications
Read Publications using
NBP1-32521 in the following applications:

  • 1 publication
  • WB
    4 publications

Reactivity Notes

Chicken (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for SUCLG2 Antibody

  • EC 6.2.1
  • EC 6.2.1.4
  • GBETA
  • GTP-specific succinyl-CoA synthetase subunit beta
  • mitochondrial
  • succinate-CoA ligase, GDP-forming, beta subunit
  • succinyl-CoA ligase, GDP-forming, beta chain, mitochondrial
  • succinyl-CoA synthetase, beta-G chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SUCLG2 Antibody (NBP1-32521)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 4 of 4.
Publications using NBP1-32521 Applications Species
Ramirez A, van der Flier WM, Herold C et al. SUCLG2 identified as both a determinator of CSF Ab1-42 levels and an attenuator of cognitive decline in Alzheimer's disease. Hum. Mol. Genet. 2014 Jul 15 [PMID: 25027320] (IHC-P, WB, Human)

Details:
SUCLG2 antibody used WB and IHC-P on human samples. IHC-P performed on paraffin tissue sections of human brain cortex (Alzheimer's disease dementia patients) with HIER using 10 mM sodium citrate buffer pH 6.0 at 100C for 20 min, a primary antibody dilution of 1 : 400 and detection with biotinylated secondary antibody- HRP-streptavidin-biotin conjugates- DAB as well as Alexa 546 labelled secondary antibody based detection methods (Figure 2A and 2B). WB performed using 1 : 500 primary dilution on lysates of frozen human brain tissue(Figure 2C) and BV2 cells transfected with two control siRNA, three siRNA directed to distinct areas of the SUCLG2 gene, or the transfection controls were probed with an antibody to SUCLG2 to demonstrate siRNA-mediated blockage of SUCLG2 expression (G. Supplementary Figure S7).
IHC-P, WB Human
Chu J. Investigation of patients diagnosed with mut methylmalonic aciduria Thesis 2016 (WB, Human) WB Human
Ou J, Peng Y, Yang W et al. ABHD5 blunts the sensitivity of colorectal cancer to fluorouracil via promoting autophagic uracil yield Nat Commun Mar 6 2019 [PMID: 30842415] (WB, Human) WB Human
Maio N, Kim KS, Holmes-Hampton G et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis Haematologica Feb 14 2019 [PMID: 30765471] (WB, Human) WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SUCLG2
Entrez
Uniprot