Sterol carrier protein 2 Antibody

Immunohistochemistry-Paraffin: Sterol carrier protein 2 Antibody [NB100-93459] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Sterol carrier protein 2 Antibody Summary

• Description: Novus Biologicals Goat Sterol carrier protein 2 Antibody (NB100-93459) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Sterol carrier protein 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KNHKHSVNNPYSQ corresponding to internal region according to NP_002970.2, NP_001180528.1, NP_001180529.1, NP_001180546.1.
• Specificity: This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2; NP_001007099.1; NP_001180529.1; NP_001180528.1; NP_001180546.1 respectively),
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 50 kDa band in human liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0 kDa band according to NP_001007099.1 and of 6.7 kDa band according to NP_002970.2. The 28 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human liver shows speckled cytoplasm staining in hepatocytes.
• Control:

  Sterol carrier protein 2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sterol carrier protein 2 Antibody

• DKFZp686C12188
• DKFZp686D11188
• NLTP
• non-specific lipid-transfer protein
• NSL-TP
• Propanoyl-CoA C-acyltransferase
• SCP-2
• SCP-CHI
• SCPX
• SCP-X
• sterol carrier protein 2EC 2.3.1.176
• Sterol carrier protein X

Background

This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. The full-length nature of all transcript variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sterol carrier protein 2 Antibody (NB100-93459)(1)

Publication using NB100-93459

• Atshaves BP, Gallegos AM, McIntosh AL et al. Sterol carrier protein-2 selectively alters lipid composition and cholesterol dynamics of caveolae/lipid raft vs nonraft domains in L-cell fibroblast plasma membranes. Biochemistry 2003-12-16 [PMID: 14661971]

Bioinformatics

• Gene Symbol: SCP2
• Entrez:
  • 6342(Human)
  • 20280(Mouse)
  • 25541(Rat)
• Uniprot:
  • P22307(Human)