Sterol carrier protein 2 Overexpression Lysate (Native)

Images

 
Western Blot: Sterol carrier protein 2 Overexpression Lysate (Adult Normal) [NBL1-15749] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sterol carrier protein 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Sterol carrier protein 2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219802 and based on accession number NM_002979. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sterol carrier protein 2 (SCP2), transcript variant 1, mRNA.
Gene
SCP2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
58.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Sterol carrier protein 2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Sterol carrier protein 2 Overexpression Lysate (Native)

  • DKFZp686C12188
  • DKFZp686D11188
  • NLTP
  • non-specific lipid-transfer protein
  • NSL-TP
  • Propanoyl-CoA C-acyltransferase
  • SCP-2
  • SCP-CHI
  • SCPX
  • SCP-X
  • sterol carrier protein 2EC 2.3.1.176
  • Sterol carrier protein X

Background

This gene encodes two proteins: sterol carrier protein X (SCPx) and sterol carrier protein 2 (SCP2), as a result of transcription initiation from 2 independently regulated promoters. The transcript initiated from the proximal promoter encodes the longer SCPx protein, and the transcript initiated from the distal promoter encodes the shorter SCP2 protein, with the 2 proteins sharing a common C-terminus. Evidence suggests that the SCPx protein is a peroxisome-associated thiolase that is involved in the oxidation of branched chain fatty acids, while the SCP2 protein is thought to be an intracellular lipid transfer protein. This gene is highly expressed in organs involved in lipid metabolism, and may play a role in Zellweger syndrome, in which cells are deficient in peroxisomes and have impaired bile acid synthesis. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. The full-length nature of all transcript variants has not been determined. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes sterol carrier protein X (SCPx) isoform 1 proprotein. The proprotein is a fusion protein whose N-terminus is evolutionary related to thiolases, and its C-terminus is identical to sterol carrier protein 2 (SCP2) isoform 1. The uncleaved proprotein has been shown to have both thiolytic and SCP2 activities. This proprotein is also partially, post-translationally cleaved to give a novel 45 kDa protein with thiolytic properties, and SCP2 isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SCP2