Human STAT5A ELISA Kit (Colorimetric)

ELISA: Human STAT5A ELISA Kit (Colorimetric) [NBP2-80280] - Samples were spiked with high concentrations of Human STAT5A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human STAT5A ELISA Kit (Colorimetric) [NBP2-80280] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.625-40 ng/mL
• Sensitivity: 0.375 ng/mL

Human STAT5A ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human STAT5A in samples. No significant cross-reactivity or interference between Human STAT5A and analogues was observed.
• Standard Curve Range: 0.625-40 ng/mL
• Sensitivity: 0.375 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.04%
• Intra-Assay: CV% < 5.72%
• Spike Recovery: 88-107%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: STAT5A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human STAT5A ELISA Kit (Colorimetric)

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: STAT5A
• Uniprot:
  • P42229(Human)
  • P42229(Human)