STAT5A Antibody (251610) [mFluor Violet 610 SE]

• Reactivity: Hu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 251610
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

STAT5A Antibody (251610) [mFluor Violet 610 SE] Summary

• Immunogen: Human STAT5a synthetic peptide
  SLDSRLSPPAGLFTSARGSLS
  Accession # NP_003143
• Specificity: Detects human STAT5a. This antibody does not cross-react with STAT5b.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT5A

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for STAT5A Antibody (251610) [mFluor Violet 610 SE]

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT5A