STAT5A Antibody (251610) [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Clone: 251610
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

STAT5A Antibody (251610) [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects human STAT5a. This antibody does not cross-react with STAT5b.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT5A Antibody (251610) [Alexa Fluor™ Plus 488]

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

STAT5a (Signal Transducer and Activator of Transcription-5a) is one of two closely related genes that belong to the STAT family of transcription factors. It is a 91 kDa cytosolic protein that contains an N-terminal domain (with an NES/Nuclear Export Signal) a coiled-coiled region (with an NLS/Nuclear Localization Signal) a DNA-binding site that recognizes a GAS (Gamma-interferon Activated Site) motif, an SH2 domain that allows for dimerization, and a C-terminal transactivation domain. STAT5a likely exists in a quiescent state as a cytoplasmic anti-parallel homodimer. Following activation of both tyrosine and non-tyrosine kinase membrane-bound receptors, STAT5a is phosphorylated, increasing its MW by some 5-6 kDa. Phosphorylated STAT5a will enter the nucleus as either a homodimer (or heterodimer with STAT5b), or as a complex with the intracellular domain of a tyrosine kinase receptor such as ErbB4. Once in the nucleus, STAT5a will form a homotetramer and bind either GAS or GAS-related sequences in gene regulatory regions. Notably, it is suggested that STAT5a may "cycle" through the nucleus without phosphorylation, a process that would seem to involve Importins alpha 3 and beta 1. STAT5a is related to STAT5b through gene duplication. They show 92% amino acid (aa) sequence identity, with the major differences existing over aa 45-56 and 773-794. The genes are not entirely redundant; STAT5a activates NDGR1 and SH2, while STAT5b regulates the Treg T cell genes, FoxP3 and CD25/IL-2R alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.