STAT5A Antibody (1B12)

Western Blot: STAT5a Antibody (1B12) [H00006776-M02] - STAT5A monoclonal antibody (M02), clone 1B12 Analysis of STAT5A expression in k-562.

Western Blot: STAT5a Antibody (1B12) [H00006776-M02] - Analysis of STAT5A expression in transfected 293T cell line by STAT5A monoclonal antibody (M02), clone 1B12.Lane 1: STAT5A transfected lysate (Predicted MW: 90.6 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: STAT5a Antibody (1B12) [H00006776-M02] - Analysis of protein-protein interactions between STAT1 and STAT5A. HeLa cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-STAT5A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: STAT5a Antibody (1B12) [H00006776-M02] - Detection limit for recombinant GST tagged STAT5A is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

STAT5A Antibody (1B12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: STAT5A (AAH27036, 1 a.a. ~ 104 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLE
• Specificity: STAT5A - signal transducer and activator of transcription 5A
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT5A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STAT5A Antibody (1B12)

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT5A
• Entrez:
  • 6776(Human)
• OMIM:
  • 601511(Human)
• Uniprot:
  • AAH27036(Human)