STAT4 Antibody (513710) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse STAT4 Monoclonal Antibody (Catalog # MAB5287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 513710
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

STAT4 Antibody (513710) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human STAT4
  Leu83-Lys162
  Accession # Q14765
• Specificity: Detects endogenous human STAT4 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT4 Antibody (513710) [Unconjugated]

• signal transducer and activator of transcription 4
• SLEB11
• STAT4

Background

Signal transduction and activator of transcription 4 (STAT4) is a 86 kDa member of the Jak/STAT signal transduction pathway. Human STAT4 is 748 amino acids (aa) in length. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo- or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 contains a SH2 domain (aa 569‑664) and a Jak1 phophorylation site at Y693. STAT4 is involved in mediating IL‑12 response in lymphocytes and regulating T helper cell differentiation, and STAT4 variants are found in patients with systemic lupus erythematosus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT4 Antibody (MAB5287)(2)

Publications using MAB5287

• Z Min, J Zhou, R Mao, B Cui, Y Cheng, Z Chen Pyrroloquinoline Quinone Administration Alleviates Allergic Airway Inflammation in Mice by Regulating the JAK-STAT Signaling Pathway Mediators of Inflammation, 2022-10-29;2022(0):1267841. 10/29/2022 [PMID: 36345503]
• F Caso, A Saviano, M Tasso, F Raucci, N Marigliano, S Passavanti, P Frallonard, R Ramonda, V Brancaleon, M Bucci, R Scarpa, L Costa, F Maione Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation Autoimmunity reviews, 2022-10-01;21(12):103207. 2022-10-01 [PMID: 36191778] (Western Blot, Human)

Bioinformatics

• Gene Symbol: STAT4
• Uniprot:
  • Q14765()